cpass sars-cov-2 neutralization antibody detection kit elisa-1  (GenScript corporation)

Journal: Frontiers in Immunology

Article Title: A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity

doi: 10.3389/fimmu.2022.1041860

Figure Lengend Snippet: Comparison of ID50 values determined by the sVNT ELISA kit and iACE2/RBD-Fc model. ID50 values of 7 vaccine serum samples and 4 convalescent plasma samples were determined by the commercial ELISA based sVNT kit and the iACE2/RBD-Fc model. A panel of raw data for determination of neutralization activity of one vaccine serum by the sVNT ELISA kit and the iACE2/RBD-Fc model was shown in (A, B) , respectively. Two sample dilution response inhibition curves generated from the data shown in Panel (A, B) were plotted and shown in (C) . ID50 values of 11 samples determined by the two assays were compared and shown in (D) . The dash line was used to indicated the serum dilution fold with an activity to inhibit 50% binding of the RBD-Fc on the ACE2 bead. p value <0.05 is represented as *.

Article Snippet: The ID50 (50% of maximal inhibitory dose) of samples were determined using an ELISA based sVNT kit (GenScript cPass SARS-CoV-2 neutralization Antibody detection kit, GenScript, China) according to instruction of the manufacturer with a minor modification.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Neutralization, Activity Assay, Inhibition, Generated, Binding Assay